Experimental data was obtained from Schreiber et al. (2010) (link). To determine the neurological effects of PBDEs, primary human fetal neural progenitor cells (hNPCs) were cultivated in the form of neurospheres and exposed to BDE-47. Neurospheres were exposed to BDE-47 over a period of 7 days, with half the medium (with a chemical concentration of 1 μM) being refreshed every second day. Exposure occurs in the Lab-Tek II Chamber slide (Thermo Fisher Scientific), which has a flat, square-based format. Culture area is reported at 0.7 cm2/well, with a total well volume of 907 μl and a working volume of 500 μl.
The medium used in these experiments was a mixture of Dulbecco’s modified Eagle medium (DMEM) and Ham’s F12 (3:1) with no supplementation with additional serum. Fischer et al. (2017) (link) determined the lipid and protein concentrations within the DMEM solution as approximately 0.2 ml/L and 0.75 (0.69–0.86) ml/L respectively. The bulk medium in the model simulations was therefore parameterized to match these volume fractions. Again, we simulated the potential influence of other dissolved organics on the distribution of the chemical following the same approach as described in Case study 1. The partitioning properties compiled for BDE-47 are summarized inTable 1 .
The medium used in these experiments was a mixture of Dulbecco’s modified Eagle medium (DMEM) and Ham’s F12 (3:1) with no supplementation with additional serum. Fischer et al. (2017) (link) determined the lipid and protein concentrations within the DMEM solution as approximately 0.2 ml/L and 0.75 (0.69–0.86) ml/L respectively. The bulk medium in the model simulations was therefore parameterized to match these volume fractions. Again, we simulated the potential influence of other dissolved organics on the distribution of the chemical following the same approach as described in Case study 1. The partitioning properties compiled for BDE-47 are summarized in
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