All chemicals and media were as
reported previously,10 (link) with the following
additions: 1,4-dithiothreitol (Carl Roth); ampicillin sodium salt
(Formedium); 14C-l -arginine (stock solution 325
mCi/mmol; NovaTec); 2-mercaptoethanol (Sigma-Aldrich); chloramphenicol
(Carl Roth); coenzyme A, free acid (Avanti Polar Lipids);d -(+)-fructose 1,6-bisphosphate trisodium salt hydrate (Sigma-Aldrich); d (+)-glucose anhydrous (Formedium); d (+)-sucrose (Formedium);
ethylenediaminetetraacetic acid disodium salt dihydrate (Sigma-Aldrich);
HEPES (Carl Roth); methanol (Sigma); n-butanol (Sigma); n-dodecyl-β-d -maltopyranoside, Anagrade (Anatrace);
oleic acid (Sigma-Aldrich); tris hydrochloride (ITW Reagents); and
TritonTM X-100 (Sigma-Aldrich).
reported previously,10 (link) with the following
additions: 1,4-dithiothreitol (Carl Roth); ampicillin sodium salt
(Formedium); 14C-
mCi/mmol; NovaTec); 2-mercaptoethanol (Sigma-Aldrich); chloramphenicol
(Carl Roth); coenzyme A, free acid (Avanti Polar Lipids);
ethylenediaminetetraacetic acid disodium salt dihydrate (Sigma-Aldrich);
HEPES (Carl Roth); methanol (Sigma); n-butanol (Sigma); n-dodecyl-β-
oleic acid (Sigma-Aldrich); tris hydrochloride (ITW Reagents); and
TritonTM X-100 (Sigma-Aldrich).
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