PDGF-BB Secretion in Macrophage-Derived Conditioned Media

Given that the phosphorylated signaling pathways were similar to that reported by others following stimulation with platelet derived growth factor (PDGF)-BB (60 (link)–63 (link)), additional studies were undertaken to quantify this analyte in Mϕ-SN and MP-SN. Mϕ-SN (U-937 derived) and MP-SN (PBMC-derived) were assayed for the secretion of PDGF-BB isoform using a commercial immunoassay platform (Meso Scale Discovery, Rockville, MD, USA; lower limit of detection of 0.29 pg/ml). PDGF-BB isoform was examined in these studies due to its role in RA pathogenesis and activation of signaling mechanisms in fibroblasts as observed in other studies (64 (link), 65 (link)). The assays were performed according to the manufacturer’s protocol and analyzed on the Meso QuickPlex SQ 120 imager (Meso Scale Discovery).

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Aripova N., Duryee M.J., England B.R., Hunter C.D., Mordeson J.E., Ryan E.M., Daubach E.C., Romberger D.J., Thiele G.M, & Mikuls T.R. (2023). Citrullinated and malondialdehyde-acetaldehyde modified fibrinogen activates macrophages and promotes an aggressive synovial fibroblast phenotype in patients with rheumatoid arthritis. Frontiers in Immunology, 14, 1203548.

Publication 2023

Meso QuickPlex SQ 120 imager

Assays Fibroblasts Immunoassay Isoform Pathogenesis Platelet derived growth factor bb Secretion Signaling pathways

Corresponding Organization : VA Nebraska Western Iowa Health Care System

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Variable analysis

independent variables

Stimulation with platelet derived growth factor (PDGF)-BB

dependent variables

Secretion of PDGF-BB isoform

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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