Spectroscopic and Chromatographic Analyses of Enzymatic Reactions

3D scans were performed on a Shimadzu RF 6000 spectrofluorophotometer. Enzyme activity by UV, fluorescence measurements and OD₆₀₀ determinations were performed using a Tecan Spark 10 M microplate reader. Enzyme activity characterization through conversions was performed by HPLC measurements, using an Agilent Technologies 1200 Series instrument equipped with autosampler. The UV irradiation of assay samples was performed in Corning 96-well Clear Flat Bottom UV-Transparent microplates using a handheld Analytik Jena UV lamp of 302 nm. The fluorescence measurement was carried out in Corning 96-well Black Flat Bottom microplates. Styrene was purchased from Sigma Aldrich and solvents (n-hexane, n-octane, dimethyl sulfoxide, acetonitrile) of HPLC grade were purchased from VWR. The synthesis of fluorogenic probe 4 was performed according to reported procedure^{47 (link)}.