Nanoparticle Characterization from PBMC

Effects and molecular constituents of EVs present in PBMC^sec were reported previously [30 (link), 32 (link)]. Nanoparticles were isolated and reconstituted in phosphate-buffered saline (PBS) as described previously [30 (link)] (Fig. 1b, c). Qualitative and quantitative assessments of nanoparticles were performed by the Molecular Neuro-Oncology, Department of Pediatrics and Adolescent Medicine and Institute of Neurology, Medical University of Vienna (Vienna, Austria), using the NanoSight NS500 instrument (Malvern Instruments, Malvern, UK). For each measurement, 500 μL of the diluted sample was loaded by the automatic pump control into the NanoSight system. The repetition and duration of captures were set manually (5 captures with 30 s each) and used for all measurements. The instrument was calibrated using 100-nm-particle reference controls provided by the instrument manufacturer.