Liposome Formulation with Tetraether Lipids

Curcumin (≥80%), cholesterol (≥99%), HEPES (≥99.5%), ethanol (absolute, ≥99.8%), D-(+)-trehalose dehydrate (≥99%) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). The lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were a gift from Lipoid GmbH (Ludwigshafen am Rhein, Germany). Fractions containing the polar tetraether lipids caldarchaeol (GDGT, approx. 10% of the extract) and calditoglycerocaldarchaeol (GDNT, approximately 90% of the extract) were extracted from the biomass of Sulfolobus acidocaldarius (Transmit GmbH, Gießen, Germany) [31 (link)]. The ends of GDNT contain phosphatidylmyoinositol and β-glucose respectively while GDGT contains phosphatidylmyoinositol and β-D-galactosyl-D-glucose on either ends respectively [32 (link)]. Ultrapure water from PURELAB^® flex 4 (ELGA LabWater, High Wycombe, UK) was used for all experiments in this study. All solvents used were of analytical or HPLC grade.

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Lehmann J., Agel M.R., Engelhardt K.H., Pinnapireddy S.R., Agel S., Duse L., Preis E., Wojcik M, & Bakowsky U. (2021). Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes. Pharmaceutics, 13(8), 1243.

Publication 2021

Curcumin cholesterol HEPES ethanol D-(+)-trehalose dehydrate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 1,2-dipalmitoyl-sn-glycero-3-phosphocholine PURELAB® flex 4

Bromide Caldarchaeol Calditoglycerocaldarchaeol Cholesterol Curcumin Dioleoyl phosphoethanolamine Ethanol Gdnt Glucose Hepes Hplc Lipids Rhein Sn glycero 3 phosphocholine Solvents Sulfolobus acidocaldarius Trehalose

Corresponding Organization : Philipps University of Marburg

Other organizations : University of Giessen

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Variable analysis

independent variables

Curcumin (≥80%)
Cholesterol (≥99%)
HEPES (≥99.5%)
Ethanol (absolute, ≥99.8%)
D-(+)-trehalose dehydrate (≥99%)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Caldarchaeol (GDGT, approx. 10% of the extract)
Calditoglycerocaldarchaeol (GDNT, approximately 90% of the extract)

dependent variables

Not explicitly mentioned

control variables

Ultrapure water from PURELAB® flex 4 (ELGA LabWater, High Wycombe, UK) was used for all experiments in this study.
All solvents used were of analytical or HPLC grade.

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