Quantifying Polyphenol Content

Total polyphenols content was evaluated using the Folin–Ciocalteu phenol reagent [25 (link)]. The absorbance at λ = 760 nm was determined at room temperature through a Cary UV/Vis spectrophotometer (Varian, Palo Alto, CA, USA). Gallic acid represented the standard. Results were expressed as μg of gallic acid equivalents (GAE)/g of the product ± standard deviation (SD). The concentration range for standard curve was between 34.02 μg and 680.04 μg.

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Fratianni F., Ombra M.N., d’Acierno A., Caputo L., Amato G., De Feo V., Coppola R, & Nazzaro F. (2021). Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria. Microorganisms, 9(8), 1694.

Publication 2021

Cary UV/Vis spectrophotometer

Folin Gallic acid Phenol Polyphenols

Corresponding Organization : University of Salerno

Other organizations : University of Molise

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total polyphenols content

control variables

Gallic acid represented the standard

controls

Positive control: Gallic acid standard curve with concentration range between 34.02 μg and 680.04 μg

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