Mouse Husbandry and Fenofibrate Diet

Mouse husbandry and procedures, including euthanasia, complied with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and were approved by the Washington University in St. Louis Institutional Animal Care and Use Committee (Protocol # 19-0950). Mouse strains included C57BL/6J (The Jackson Laboratory, Stock #00664), BKS.Cg-Dock7^m +/+ Lepr^db/J (“db/db”, The Jackson Laboratory, Stock #00642), Tg(Slc1a3-cre/ERT)1Nat/J (“GLAST-CreER”, The Jackson Laboratory, Stock #012586), and B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (“tdTomato” The Jackson Laboratory, Stock #007914) [20 (link),21 (link),22 (link)]. For in vivo luciferase assays, we used a peroxisome proliferator response element luciferase transgenic mouse on the C57/BL/6J background (repTOP PPRE-Luc, Charles River), as previously described [23 (link),24 (link)]. After weaning, all mice were maintained on a standard chow diet until 3 months of age. At 3 months, mice were maintained on standard chow or transitioned to a custom-milled standard chow diet supplemented with 0.2% w/w fenofibrate (Envigo Teklad rodent diet) as previously described [25 (link)].