Cell Signaling Pathway Inhibitors Assay

Anisomycin, SB203580 [45 (link)], SP600125, and ferrostatin-1 [46 (link)] were purchased from MCE (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO). Cells were treated with anisomycin at the indicated concentration or coincubated with SB203580 (20 μM) or ferrostatin-1 (4 μM). Commercially available antibodies were used in this study. The anti-tubulin antibody was obtained from InTech (Shanghai, China), while anti-c-Myc (#18583), CD133 (#64326), Nanog (#4903), EpCAM (#2929), caspase-3 (#14220), Bcl-2 (#15071), Bax (#5023), N-cadherin (#13116), E-cadherin (#3195), vimentin (#5741), α-smooth muscle actin (α-SMA, #19245), p38 MAPK (#8690), phospho-p38 MAPK (p-p38 MAPK, #9216), histone H3 (#4499), phospho-histone H3 (Ser10) (p-H3S10, #53348), phospho-histone H3 (Ser28) (p-H3S28, #9713), NRF2 (#12721), SLC7A11 (#12691), FTH1 (#4393), and NCOA4 (#66849) antibodies were purchased from Cell Signaling Technology (Boston, USA). Anti-CD24 (ab31622) antibody was provided by Abcam (Cambridge, UK).

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Chen W., Yang W., Zhang C., Liu T., Zhu J., Wang H., Li T., Jin A., Ding L., Xian J., Tian T., Pan B., Guo W, & Wang B. (2022). Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10. Oxidative Medicine and Cellular Longevity, 2022, 6986445.

Publication 2022

EpCAM caspase-3 Bcl-2 Bax N-cadherin E-cadherin vimentin α-smooth muscle actin (α-SMA, p38 MAPK phospho-p38 MAPK (p-p38 MAPK, histone H3 SLC7A11 NCOA4

Actin Anisomycin Anti antibody Antibodies Antibody Bcl 2 Caspase 3 Cells Dimethyl sulfoxide E cadherin Epcam Ferrostatin 1 Histone h3 N cadherin Nrf2 P38 mapk Sb203580 Smooth muscle Sp600125 Tubulin Vimentin

Corresponding Organization : Zhongshan Hospital of Xiamen University

Other organizations : Shanghai University of Traditional Chinese Medicine

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Variable analysis

independent variables

Anisomycin
SB203580
SP600125
Ferrostatin-1

dependent variables

Cell response

control variables

Cell culture conditions
Antibody sources

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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