Invasive Lung Function Measurement

Mice were intraperitoneally (i.p.) anaesthetized with a mix containing Ketamine (Vetoquinol S.A., Lure Cedex, France; 125 mg/kg) and Medetomidine (Pfizer, Capelle a/d Ijssel, Netherlands; 0.4 mg/kg). Lung function was assessed using EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, Zwijndrecht, The Netherlands) (0–25 mg/mL, 10% puff for 10 sec.). Data are presented as average lung resistance (R_L) in cm H₂O/mL*sec⁻¹ [28 (link)].

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Verheijden K.A., Willemsen L.E., Braber S., Leusink-Muis T., Delsing D.J., Garssen J., Kraneveld A.D, & Folkerts G. (2015). Dietary galacto-oligosaccharides prevent airway eosinophilia and hyperresponsiveness in a murine house dust mite-induced asthma model. Respiratory Research, 16(1), 17.

Publication 2015

Ketamine Medetomidine EMKA invasive measurement of dynamic resistance acetyl-β-methyl-choline chloride

Cedex Choline chloride Ketamine Lung Lung function Medetomidine Methacholine Mice

Corresponding Organization : Utrecht University

Other organizations : FrieslandCampina (Netherlands)

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Variable analysis

independent variables

Increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, Zwijndrecht, The Netherlands) (0–25 mg/mL, 10% puff for 10 sec.)

dependent variables

Lung function assessed using EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France)

control variables

Mice anaesthetized with a mix containing Ketamine (Vetoquinol S.A., Lure Cedex, France; 125 mg/kg) and Medetomidine (Pfizer, Capelle a/d Ijssel, Netherlands; 0.4 mg/kg)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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