Controlled ovarian hyper-stimulation was performed using a short-acting GnRH agonist long protocol. Recombinant follicle-stimulating hormone (Merck Serono) was started at least 14 days after the downregulation of GnRH agonist for complete suppression of estradiol from 75 to 300IU/d. Ovulation was induced with human chorionic gonadotropin, and approximately 36 hours later, oocyte retrieval was performed under transvaginal ultrasonographic guidance. Fertilization was carried out using the standard IVF technique; if male infertility or fertilization failure occurred, oocytes were inseminated by ICSI. Embryo transfer was mostly performed using cleaving stage embryos (day 3). If a patient was at risk of ovarian hyperstimulation syndrome, the embryo was vitrified and transferred to a subsequent substituted cycle.
Protocol detail
Controlled Ovarian Hyperstimulation Protocol for IVF
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Downregulation
Embryo
Embryo transfer
Estradiol
Fertilization
Follicle stimulating hormone
Gnrh
Human chorionic gonadotropin
Icsi
Male infertility
Oocyte retrieval
Oocytes
Ovarian hyperstimulation syndrome
Ovarian stimulation
Ovulation
Patient
Corresponding Organization : Jiaxing University
Other organizations : First Hospital of Jiaxing
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Variable analysis
independent variables
- Controlled ovarian hyper-stimulation using a short-acting GnRH agonist long protocol
- Recombinant follicle-stimulating hormone (Merck Serono) started at least 14 days after the downregulation of GnRH agonist for complete suppression of estradiol from 75 to 300IU/d
- Number of oocytes retrieved
- Fertilization rate
- Embryo development and quality
- Incidence of ovarian hyperstimulation syndrome
- Transvaginal ultrasonographic guidance for oocyte retrieval
- Standard IVF technique for fertilization
- ICSI for cases of male infertility or fertilization failure
- Embryo transfer mostly using cleaving stage embryos (day 3)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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