The Annexin V-APC/7-AAD Apoptosis Kit (Abnova, #KA3808) was used for the apoptosis analysis by flow cytometry. Cells were harvested, counted, and washed in the binding buffer. Cells were then stained with annexin V-APC and 7-ADD, and analyzed by a flow cytometer (BD Biosciences, San Jose, CA) as recommended by the manufacturer. Results were analyzed by the FlowJo software (version 10.1, Ashland, OR). ALDH assays were performed as described previously.21 (link) All experiments were performed in triplicate.
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Quantifying Apoptosis by Flow Cytometry
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Corresponding Organization : Montefiore Medical Center
Other organizations : Peking University People's Hospital, Peking University, University of California, Irvine Medical Center, The University of Texas MD Anderson Cancer Center
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Variable analysis
independent variables
- Abnova Annexin V-APC/7-AAD Apoptosis Kit
- Apoptosis analysis by flow cytometry
- ALDH assays
- Binding buffer
- Annexin V-APC staining
- 7-AAD staining
- Flow cytometer (BD Biosciences)
- Positive control: Not specified
- Negative control: Not specified
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