Pyrosequencing of five CpG sites from MGMT promoter was performed with the PyroMark Q24 CpG MGMT kit (ref. 970032) (Qiagen, France) on a PyroMarkQ24 system (Qiagen), as per manufacturer's recommendation. When the methylation percentage of MGMT (ie the mean value of the five CpG sites tested) was above 8%, the sample was classified as methylated [15 (link),16 (link)].
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