All experiments were performed in accordance with UK Home Office regulations. Embryos were grown at 28.5°C as previously described.38 Lines used were AB (considered to be wildtype), masterblind (mbl),39 (link), Tg[elavl3:gfp],40 (link)
Tg[dusp6:d2eGFP],41 (link)
Tg[hsp70l:dkk1b-gfp],42 (link)
Tg[hsp70:ca-fgfr1],43 (link)
Tg[hsp70:gal4],44 (link)
Tg[dlx5a/6a:eGFP],45 (link)
Tg[UAS:HA-β-catenin],34 (link)
Tg[TOPdGFP].46 (link)
Heat shock induction was performed by moving embryos to 37°C for 2 hours at 16.5 hours post fertilization (hpf). Pharmacological treatments were performed by applying either SU5402 (Sigma), BIO (Invitrogen) or IWR-1 (Merck) diluted in embryo medium as previously described.34 (link) For all experimental conditions, a minimum of n = 10 embryos were used; for each individual experiment containing multiple conditions, embryos from the same clutch were used to minimise variation in developmental stage.
Tg[dusp6:d2eGFP],41 (link)
Tg[hsp70l:dkk1b-gfp],42 (link)
Tg[hsp70:ca-fgfr1],43 (link)
Tg[hsp70:gal4],44 (link)
Tg[dlx5a/6a:eGFP],45 (link)
Tg[UAS:HA-β-catenin],34 (link)
Tg[TOPdGFP].46 (link)
Heat shock induction was performed by moving embryos to 37°C for 2 hours at 16.5 hours post fertilization (hpf). Pharmacological treatments were performed by applying either SU5402 (Sigma), BIO (Invitrogen) or IWR-1 (Merck) diluted in embryo medium as previously described.34 (link) For all experimental conditions, a minimum of n = 10 embryos were used; for each individual experiment containing multiple conditions, embryos from the same clutch were used to minimise variation in developmental stage.
