Western blotting of chimeric MAbs was carried out as described previously63 (link), except that HRP-conjugated goat anti-mouse IgG (diluted 1:10,000; Sigma, USA) and HRP-conjugated rabbit anti-human IgG (diluted 1:10,000; Abcam, USA) were used for detection. Note that anti-human IgG or anti-mouse IgG secondary antibodies (HRP conjugate) used in this study recognize the constant domains of both heavy and light chains.
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