Serum Exosome Isolation and Characterization

The serum exosomes^{16 (link)–18 (link)} were isolated using supercentrifugation (L-90KL-80XP, Beckman Coulter, California, America). Briefly, the serum samples were first centrifuged at 2000 g for 30 min to remove died cells and debris, then centrifuged at 100,000 g for 70 min, supernatant was discarded. The sediment was washed by 1 ml phosphate-buffered saline (PBS) and were centrifuged at 100,000 g for 70 min^{19 (link)}, supernatant was discarded and the remaining was mainly serum exosomes. Finally, serum exosomes were resuspended with 200μL PBS for identification and RNA extraction.

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Wang F., Yang B., Qiao J., Bai L., Li Z., Sun W., Liu Q., Yang S, & Cui L. (2023). Serum exosomal microRNA-1258 may as a novel biomarker for the diagnosis of acute exacerbations of chronic obstructive pulmonary disease. Scientific Reports, 13, 18332.

Publication 2023

L-90KL-80XP

Cells Exosomes Phosphate Saline Serum

Corresponding Organization :

Other organizations : Peking University Third Hospital, Peking University

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Variable analysis

independent variables

Isolation of serum exosomes using supercentrifugation

dependent variables

Identification and RNA extraction of serum exosomes

control variables

Centrifugation speed and duration
Washing of sediment with phosphate-buffered saline (PBS)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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