Strains of the green microalgae B. braunii SAG 30.81, S. obliquus SAG 276-3a, N. conjuncta SAG 78.80, N. terrestris UTEX B. 947, and N. texensis SAG 99.80 originating from the SAG Culture Collection of Algae and the UTEX Culture Collection of Algae were inoculated from solid into sterile liquid Kessler’s medium to obtain a sufficiently large quantity of algal biomass required for the experiments. Preliminary semi-continuous cultures were run under light (Osram L58W/765 cool daylight) and temperature 24 ± 1 °C for 60 days until biomass suitable for the experiments was obtained. The intensity of photosynthetically active light (PPFD) was 60 μmol m−2 s−1.
The biomass obtained was used (1) for determination of the relationship curves between the optical density of algal culture measured with the spectrophotometric method (Unicam Helios, UK) at the 650-nm wavelength and the dry weight (determined with the weighing method) of algae growing under the conditions specified above, and (2) as an inoculum for the growth experiments.
The biomass obtained was used (1) for determination of the relationship curves between the optical density of algal culture measured with the spectrophotometric method (Unicam Helios, UK) at the 650-nm wavelength and the dry weight (determined with the weighing method) of algae growing under the conditions specified above, and (2) as an inoculum for the growth experiments.
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