Antibodies for Nucleolar Protein Analysis

Mouse monoclonal antibodies against NPM1 (clone FC82291, B0556, Sigma-Aldrich, St. Louis, MO, USA), α-tubulin (T6199, Sigma-Aldrich), β-actin (A5441, Sigma-Aldrich) and rabbit polyclonal antibodies against GFP (60–011, BioAcademia, Osaka, Japan) and histone H3 (#9715, Cell Signaling, Danvers, MA, USA), were purchased commercially. Mouse monoclonal antibodies against NPM (clone 9–2.6)^{30 (link)} and G196^{32 (link)} were described previously. Rabbit polyclonal antibody against NPM1c was raised using a synthetic peptide corresponding to the C-terminal sequence of NPM1c type A (DLCLAVEEVSLRK). Horseradish peroxidase-conjugated goat F(ab’)₂ anti-mouse IgG (H+L) (#710–133, Rockland Immunochemicals, Limerick, ME, USA), goat anti-rabbit IgG (111–035–003, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and Alexa 594-conjugated goat anti-mouse IgG (H+L) (A-11032, ThermoFisher Scientific, Waltham, MA, USA) were purchased commercially.