HEK-Blue hTLR4 Cell-Based Assay

Human embryonic kidney (HEK)-Blue^TM hTLR4 (Invivogen) cells were prepared in HEK-Blue detection medium (Invivogen) to enable real-time detection of SEAP. The SEAP assay was performed as previously described [54 (link)]. Briefly, peptides were prepared by concentration in 96-well plates, and cells were added at 2.5 × 10⁴ cells/well. After 1 h, they were stimulated with LPS (O111:B4) (20 ng/mL), followed by 16 h incubation, and the 620 nm absorbance was measured.

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Lee H., Kim B., Kim M., Yoo S., Lee J., Hwang E, & Kim Y. (2023). Characterization of the Antimicrobial Activities of Trichoplusia ni Cecropin A as a High-Potency Therapeutic against Colistin-Resistant Escherichia coli. Pharmaceutics, 15(6), 1752.

Publication 2023

HEK)-BlueTM hTLR4 HEK-Blue detection medium

Assay Cells Embryonic Human Kidney Peptides

Corresponding Organization : Konkuk University

Other organizations : Korea Basic Science Institute

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Variable analysis

independent variables

Peptides

dependent variables

SEAP activity (measured by 620 nm absorbance)

control variables

Cell density (2.5 × 10^4 cells/well)
Incubation time (16 h)
LPS concentration (20 ng/mL)

controls

Positive control: LPS (O111:B4) stimulation

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