β-glucan exposure on C. albicans cells was quantified by flow cytometry27 (link),28 . Briefly, exponential cells were incubated for 5 h under the conditions specified and then fixed with 50 mM thimerosal (Sigma-Aldrich, Dorset, UK). The cells were then stained with Fc-Dectin-1 and anti-human IgG conjugated to Alexafluor 488 (Invitrogen), and the fluorescence of 10,000 events acquired using a BD Fortessa flow cytometer. The gating strategy and axis scales, which remained unchanged throughout, are presented in Supplementary Fig. 4 . Median fluorescence intensities (MFI) were determined using FlowJo v.10 software. Each cytometry plot is representative of at least three independent biological replicates.
To examine chitin levels in C. albicans, thimerosal-fixed cells were stained for 60 min in the dark with 10 µg mL−1 CFW (Sigma-Aldrich, Dorset, UK). These cells were imaged by fluorescence microscopy (above) and their fluorescence was quantified by flow cytometry without further staining (above: Supplementary Fig.4 ).
To examine chitin levels in C. albicans, thimerosal-fixed cells were stained for 60 min in the dark with 10 µg mL−1 CFW (Sigma-Aldrich, Dorset, UK). These cells were imaged by fluorescence microscopy (above) and their fluorescence was quantified by flow cytometry without further staining (above: Supplementary Fig.
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