The zmnac17-1 and zmnac17-2 mutants were collected from the maize EMS mutant library [41 (link)]. The B73 wild type from the same library was used as a control. All materials were propagated in Jiaozhou, Shandong (36°27′ N, 120°03′ E) in summer and in Ledong, Hainan (18°45′ N, 109°10′ E) in winter. Genotyping was evaluated using Sanger sequencing. DNA from leaves was extracted using the CTAB method. Primers were designed using the NCBI website (Supplementary Table S1 ). PCR was performed using 2× Taq PCR StarMix with Loading Dye (A012, GenStar). Sanger sequencing was performed by Sangon Biotech Co., Ltd (Shanghai, China).
zmnac17-1, zmnac17-2, and B73 were used for mesocotyl elongation analysis. zmnac17-1 and B73 were used for physiological and biochemical analyses, hormone content determination, and RNA sequencing analysis. Seeds were sown in a 54 × 28 × 9 cm high-footed seedling tray in the dark. Each hole was filled with vermiculite and then fully watered, and the seedlings were grown in a dark incubator (25 °C). The mesocotyl lengths of the 7-day-old seedlings were measured according to previously published methods [5 (link)]. At least 15 individual seedlings for each genotype were analyzed.
zmnac17-1, zmnac17-2, and B73 were used for mesocotyl elongation analysis. zmnac17-1 and B73 were used for physiological and biochemical analyses, hormone content determination, and RNA sequencing analysis. Seeds were sown in a 54 × 28 × 9 cm high-footed seedling tray in the dark. Each hole was filled with vermiculite and then fully watered, and the seedlings were grown in a dark incubator (25 °C). The mesocotyl lengths of the 7-day-old seedlings were measured according to previously published methods [5 (link)]. At least 15 individual seedlings for each genotype were analyzed.
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