Immunocytochemical Analysis of Cell Markers

For immunocytochemistry, cells were processed as already reported [28 (link),29 (link)]. The primary mouse antibody against E-cadherin, N-cadherin, vimentin, VEGF, actin and tubulin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). The secondary antibodies Alexa Fluor^® 568 conjugate goat anti-mouse was acquired from Thermo Fisher Scientific (Waltham, MA, USA). Nuclei were stained using DAPI 0.2 µg/mL (Sigma). Fluorescence was detected by using a fluorescence microscopy (Leica DM6000). All the images, processed with LAS-X software, are representative fields of three independent experiments.

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Iacopetta D., Fazio A., La Torre C., Barbarossa A., Ceramella J., Francomano F., Saturnino C., El-Kashef H., Alcaro S, & Sinicropi M.S. (2022). Annona cherimola Mill. Leaf Extracts Affect Melanoma Cells Growth and Progression. Foods, 11(16), 2420.

Publication 2022

primary mouse antibody E-cadherin N-cadherin vimentin VEGF Alexa Fluor® 568 conjugate goat anti-mouse DAPI DM6000

Actin Alexa 568 Antibodies Antibody Cadherin Cells Dapi Fluorescence Fluorescence microscopy Goat Immunocytochemistry Mouse N cadherin Nuclei Tubulin Vegf Vimentin

Corresponding Organization : University of Calabria

Other organizations : University of Bari Aldo Moro, University of Basilicata, Assiut University, Magna Graecia University

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Variable analysis

independent variables

Primary mouse antibody against E-cadherin, N-cadherin, vimentin, VEGF, actin and tubulin

dependent variables

Fluorescence detected by using a fluorescence microscopy

control variables

Cells were processed as already reported [28, 29]
Nuclei were stained using DAPI 0.2 µg/mL

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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