Genome assemblies have been deposited on NCBI GenBank. The NCBI GenBank accession numbers for the leatherback turtle assembly (rDerCor1) are GCF_009764565.3 and GCA_009762595.2 for the annotated primary and original alternate haplotypes in BioProject PRJNA561993, and for the green turtle assembly (rCheMyd1) are GCF_015237465.2 and GCA_015220195.2 for primary and alternate haplotypes respectively in BioProject PRJNA561941. The raw data used for assemblies are available on the Vertebrate Genome Ark (https://vgp.github.io/genomeark/). The leatherback turtle data generated for the purpose of assembly annotation was deposited in the SRA under accession numbers SRX8787564-SRX8787566 (RNA-Seq) and SRX6360706-SRX6360708 (ISO-Seq). Green turtle data generated for annotation were deposited in SRA under accessions SRX10863130-SRX10863133 (RNA-Seq) and as SRX11164043-SRX11164046 (ISO-Seq). The NovaSeq 6000 DNA-Seq data for the green turtle resequencing, including raw reads, are deposited in NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject ID: PRJNA449022. All scripts used for downstream analyses following genome assembly and annotation have been deposited on GitHub under repository https://github.com/bpbentley/sea_turtle_genomes.