After a 12 h overnight fast, participants arrived at the NIH Clinical Center at 7AM. Weight, height, waist circumference (WC) and blood pressure (BP) were measured. Hypertension (HT) was defined as SBP ≥ 130 mmHg and/or DBP ≥ 80 mmHg25 (link). Obesity was defined as BMI ≥ 30 kg/m226 . WC was measured at the superior border of the iliac crest at the end of expiration27 (link), and the mean of three values was recorded.
Baseline blood samples were obtained for fasting glucose, insulin, glycated hemoglobin A1c (HbA1c), high-sensitivity cardiac troponin T (hs-cTnT), amino-terminal pro-brain natriuretic peptide (NT-proBNP), fibrinogen, high-sensitivity C-reactive protein (hsCRP), cholesterol, triglycerides and HDL cholesterol. Estimated glomerular filtration rate (eGFR) was calculated according to the chronic kidney disease epidemiology (eGFR-CKD-EPI) collaboration formula28 (link). Post-Glucola consumption (Trutol 75, Custom Laboratories) blood samples were taken at 0.5 h, 1 h, 2 h to determine glucose and insulin concentrations, which were used to calculate the Matsuda Index.
After the OGTT, a computerized tomographic (CT) scan (Siemens and Somatom Force Scanner) with adipose windows designed to measure visceral adipose tissue (VAT), was performed29 (link).
Baseline blood samples were obtained for fasting glucose, insulin, glycated hemoglobin A1c (HbA1c), high-sensitivity cardiac troponin T (hs-cTnT), amino-terminal pro-brain natriuretic peptide (NT-proBNP), fibrinogen, high-sensitivity C-reactive protein (hsCRP), cholesterol, triglycerides and HDL cholesterol. Estimated glomerular filtration rate (eGFR) was calculated according to the chronic kidney disease epidemiology (eGFR-CKD-EPI) collaboration formula28 (link). Post-Glucola consumption (Trutol 75, Custom Laboratories) blood samples were taken at 0.5 h, 1 h, 2 h to determine glucose and insulin concentrations, which were used to calculate the Matsuda Index.
After the OGTT, a computerized tomographic (CT) scan (Siemens and Somatom Force Scanner) with adipose windows designed to measure visceral adipose tissue (VAT), was performed29 (link).
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