Drugs used in this study included nicotine tartrate (MP Biomedicals, LLC, Solon, OH), RO5263397 (synthesized at Research Triangle Institute, purity > 98%) and mecamylamine hydrochloride (Sigma). nicotine tartrate was dissolved in 0.9% physiological saline, and the pH was adjusted to 7.2–7.4 prior to injection. RO5263397 was dissolved in a mixture of 1 part absolute ethanol, 1 part Emulphor-620 (Rhodia) and 18 parts physiologic saline.36 (link) The dose of RO5263397 (intraperitoneally, i.p.; 5.6 mg/kg) used in these experiments was selected based on our previous findings in which there was no effect on general locomotor activity.30 (link),37 (link) Mecamylamine hydrochloride was dissolved in saline and administered i.p. (1.5 mg/kg).
Protocol detail
Pharmacological Evaluation of Nicotinic Agents
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
0 9 saline
Absolute ethanol
Drugs
Emulphor
Locomotor activity
Mecamylamine
Nicotine tartrate
Physiologic
Ro5263397
Saline
Solon
Corresponding Organization : RTI International
Top 5 similar protocols
Variable analysis
independent variables
- Nicotine tartrate
- RO5263397
- Mecamylamine hydrochloride
- Not explicitly mentioned
- Physiological saline
- Ethanol
- Emulphor-620
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!