NeuroHeal (NH) is composed of acamprosate calcium (Aca) and Ribavirin (Rib) compounds [7 (link)]. Aca, Rib, Ex-527, and nicotinamide (NAM) (Sigma-Aldrich, Saint Louis, MO, USA), and 3-Methyladenine (3MA) (Tocris) for in vitro studies were diluted in sterile H2O or DMSO. Aca, Rib, Ex-527, and NAM were added at a final concentration of 1 μM, 55 μM, 10 µm, 5 mM, and 10 μM, respectively, and were mixed with the culture medium.
In vivo treatment with NH consists of Aca (Merck) and Rib (Normon) pills grounded into a fine powder and dissolved in drinking water at 2.2 mM and 1 mM, respectively. In some experiments, NAM was dissolved and added also in the drinking water at 5 mM. We changed the tap water every 3 days and freshly added the drug treatment on a daily basis. DMOG (Tocris), dissolved in DMSO, was injected daily at 20 mg/kg.
In vivo treatment with NH consists of Aca (Merck) and Rib (Normon) pills grounded into a fine powder and dissolved in drinking water at 2.2 mM and 1 mM, respectively. In some experiments, NAM was dissolved and added also in the drinking water at 5 mM. We changed the tap water every 3 days and freshly added the drug treatment on a daily basis. DMOG (Tocris), dissolved in DMSO, was injected daily at 20 mg/kg.
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