Antibody-Conjugated Agarose Bead Assay

Anti-Flag antibody, anti-Myc antibody and anti-HA antibody-conjugated agarose beads were purchased from Sigma-Aldrich. The experimental procedures including Western blot and coimmunoprecipitation analysis have been described previously [79 (link)]. The Fuji Film LAS4000 mini luminescent image analyzer was used for photographing the blots. Multigauge V3.0 was used for quantifying the protein levels based on the band density obtained in the Western blot analysis.

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Li Z., Li J., Li Z., Song Y., Wang Y., Wang C., Yuan L., Xiao W, & Wang J. (2024). Zebrafish mylipb attenuates antiviral innate immunity through two synergistic mechanisms targeting transcription factor irf3. PLOS Pathogens, 20(5), e1012227.

Publication 2024

Anti-Flag antibody anti-Myc antibody anti-HA antibody-conjugated agarose beads

Corresponding Organization : Shanghai Zhangjiang Laboratory

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Variable analysis

independent variables

Anti-Flag antibody
Anti-Myc antibody
Anti-HA antibody-conjugated agarose beads

dependent variables

Protein levels based on band density obtained in the Western blot analysis

control variables

Experimental procedures including Western blot and coimmunoprecipitation analysis

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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