Quantitative RT‐PCR (qRT‐PCR) was performed using a Rotor‐Gene Q 5plex HRM Platform (Qiagen). Each PCR reaction was carried out as described by Fiorilli et al. (2015 (link)). All reactions were performed on at least four biological and two technical replicates. The transcript levels of rice OsPT11 (Güimil et al., 2005 ), OsLysM (Fiorilli et al., 2015 (link)), OsCCD8 and OsMAX1 (Wang et al., 2019 ), and OsD14L (Gutjahr et al., 2015 (link)) and OsSMAX1 (Choi et al., 2020 ) and fungal housekeeping Fm18S (Balestrini et al., 2007 (link)) were normalized using the OsRubQ1 housekeeping gene (Table S3).
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