Immunofluorescence was used to detect F4/80, CD45.1, and CD45.2 as described by us previously [13 (link)]. Briefly, frozen livers were cut into 8 μm sections, fixed in 4% formalin for 10 minutes, followed by blocking in 10% goat serum. The sections were then incubated with Alexa Fluor-488 anti-mouse CD45.1 antibody (Biolegend) diluted 1:100, Alexa Fluor-488 anti-mouse CD45.2 antibody (Biolegend) diluted 1:100, or rat anti-F4/80 antibody (Bio-Rad, Hercules, CA) diluted 1:500. The sections were then incubated with goat anti-rat secondary antibody conjugated to Alexa Fluor-594 diluted 1:500 (Thermo-Fisher Scientific).
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Immunofluorescence Analysis of Liver Cells
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Corresponding Organization : Michigan State University
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Variable analysis
independent variables
- Alexa Fluor-488 anti-mouse CD45.1 antibody (Biolegend) diluted 1:100
- Alexa Fluor-488 anti-mouse CD45.2 antibody (Biolegend) diluted 1:100
- Rat anti-F4/80 antibody (Bio-Rad, Hercules, CA) diluted 1:500
- Not explicitly mentioned
- Frozen livers were cut into 8 μm sections
- Sections were fixed in 4% formalin for 10 minutes
- Sections were blocked in 10% goat serum
- Sections were incubated with goat anti-rat secondary antibody conjugated to Alexa Fluor-594 diluted 1:500 (Thermo-Fisher Scientific)
- Positive control: Not specified
- Negative control: Not specified
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