Induction of Synovial Inflammation and Lameness

Synovial inflammation and forelimb lameness was induced in all sixteen horses using a previously described model of LPS induced synovitis [3 (link), 5 (link), 11 (link)]. Briefly, LPS (E.coli O55:B5; Sigma-Aldrich, St. Louis, MO) was prepared in a sterile manner, at a concentration of 100 ng/mL in Dulbecco PBS solution. The area surrounding the right antebrachiocarpal joint was clipped and prepared for injection and localized inflammation induced by sterile injection of 100 ng (1 mL) of LPS. Additional doses of LPS were administered in the same manner on days 4 and 9.

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Knych H.K., Weiner D., Harrison L, & McKemie D.S. (2022). Pharmacokinetics and pharmacodynamics of intra-articular isoflupredone following administration to horses with lipopolysaccharide-induced synovitis. BMC Veterinary Research, 18, 436.

Publication 2022

LPS (E.coli O55:B5;

E coli Forelimb Horses Inflammation Joint Sterile Synovitis

Corresponding Organization :

Other organizations : West Health

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Variable analysis

independent variables

Lipopolysaccharide (LPS) from E. coli O55:B5 at a concentration of 100 ng/mL
Injection of 100 ng (1 mL) of LPS into the right antebrachiocarpal joint
Additional doses of LPS administered on days 4 and 9 in the same manner

dependent variables

Synovial inflammation
Forelimb lameness

control variables

Sterile preparation of the injection site
Injection volume (1 mL)

controls

Positive control: No control group mentioned, but the study references previously described models of LPS-induced synovitis [3, 5, 11]
Negative control: No control group mentioned

Annotations

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