FTIR Analysis of Piper betle Extract

The FTIR Spectrometer (Alpha P; Bruker, Germany) was used, which was interlinked to desktop operated Windows system to connect with OPUS Data Collection software (Version 7.5; Bruker, Germany). Prior to the analysis, the attenuated total reflectance (ATR) plate was cleaned thoroughly by using ethanol (70%), after which it was dried with a soft tissue. For loading the sample, one drop of P. betle extract was placed onto the crystal of the ATR plate, while keeping the anvil arm in upright position by active rotation. The FTIR spectra were obtained with the addition of 100 scans in the range of 4000–400 cm⁻¹ at a resolution of 4 cm⁻¹ [97 (link)].