Senescent cells (SCs) were detected used the fluorescent senescence-associated-β-galactosidase assay (19 (link), 20 (link)). Callus cells or cultured CaMPCs were treated with 100 μM 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-galactopyranoside (DDAOG) Thermo Fisher Scientific, cat#: D-6488) for 50 minutes and subjected to flow cytometry for 7-hydroxy-9H(I,3-dichloro-9,9-dimethylacridin-2-one) signal.
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