The DIA-MS data were analyzed using the Spectronaut Pulsar (Biognosys, Switzerland) with the default settings. All of the results were filtered with a Q-value cutoff of 0.01 (corresponding to an FDR of 1%). Proteins identified in more than 50% of the samples in at least one subgroup were retained for further analysis. Missing values were imputed based on the k-nearest neighbor method or by the minimum value (details provided in Supplementary Figure S1A ).
Raw proteomics data were log10 transformed and then centralized. Student’s t-test was used, and the software was R (version 4.1.1). Any differential proteins that fulfilled all of the limitations were considered significant: 1) p-value <0.05; and 2) Fold change ≥2.
Raw proteomics data were log10 transformed and then centralized. Student’s t-test was used, and the software was R (version 4.1.1). Any differential proteins that fulfilled all of the limitations were considered significant: 1) p-value <0.05; and 2) Fold change ≥2.
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