Depletion of CD90+ ILCs in Mice

Depletion of CD90⁺ ILCs was performed as previously described, with some modifications (36 (link)). Two hundred and fifty micrograms of a mAb to CD90.2 or an isotype control rat IgG2b (BioXCell, West Lebanon, NH) was given by means of intra-peritoneal injection a total of three times at 3-day intervals. Intestinal ECs and LP cells were collected 2 days after the final injection.

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Goto Y., Obata T., Kunisawa J., Sato S., Ivanov I.I., Lamichhane A., Takeyama N., Kamioka M., Sakamoto M., Matsuki T., Setoyama H., Imaoka A., Uematsu S., Akira S., Domino S.E., Kulig P., Becher B., Renauld J.C., Sasakawa C., Umesaki Y., Benno Y, & Kiyono H. (2014). Innate lymphoid cells regulate intestinal epithelial cell glycosylation. Science (New York, N.Y.), 345(6202), 1254009.

Publication 2014

isotype control rat IgG2b

Cd90 Cells Igg2b Intestinal Intra peritoneal injection Isotype

Corresponding Organization :

Other organizations : The University of Tokyo, Japan Science and Technology Agency, RIKEN BioResource Research Center, National Institute of Biomedical Innovation, Health and Nutrition, Columbia University Irving Medical Center, Nippon Institute for Biological Science, Yakult Central Institute, Chiba University, Osaka University, University of Michigan–Ann Arbor, University of Zurich, Ludwig Cancer Research, Chiba University Hospital, RIKEN Nishina Center

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Variable analysis

independent variables

Depletion of CD90+ ILCs

dependent variables

Collection of intestinal ECs and LP cells

control variables

Injection of isotype control rat IgG2b

controls

Positive control: Injection of a mAb to CD90.2
Negative control: Injection of isotype control rat IgG2b

Annotations

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