The study was conducted in an L-format with automated Glan-Thompson polarizing prisms controlled by FluorEssence software (Horiba). The anisotropy values for each dye and dye-conjugate were determined at relatively low concentrations with absorption below 0.2 a.u. to avoid aggregation of the dye or agglomeration of the proteins. The details of the procedure were published previously (8 (link)).
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Fluorescence Anisotropy Measurements
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Other organizations : Washington University in St. Louis
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Variable analysis
independent variables
- Type of dye or dye-conjugate
- Anisotropy values
- Dye or dye-conjugate concentration (kept below 0.2 a.u. absorption to avoid aggregation or agglomeration)
- Positive control: Not specified
- Negative control: Not specified
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