Standard and samples were separated by HPAEC and detected by pulsed amperometric detection (Dionex) using a CarboPack PA10 (2 × 250 mm) column (LCPackings). Acidic GlcA and GalA were eluted by 10 mM NaOH and 10 mM CH3COONH4 with 1 ml/min flow rate at a retention time of 25 min.
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