Polysaccharide Extraction from Black Beans

The specific separation and purification process was shown in Supplementary Materials Figure S1. Tersely, the dried Black bean was decorticated, smashed, defatted, and then extracted 3 times using the distilled water at 80 °C for two hours. The supernatant was collected and precipitated with alcohol. The collected sediment was again dissolved by the distilled water to be dialyzed and lyophilized to get the Black bean crude extraction. Finally, a polysaccharide was separated from the crude extraction by using distilled water as the eluent through the DEAE-52 cellulose (Cl⁻ form) and Sephadex G-100 columns.