Quantifying Early Endothelial Progenitor Cells

From 45 mL of intravenous blood, measures of EPC-CFU were obtained by isolating early EPCs using Ficoll Paque and seeding 5 million cells on 6-well Fibronectin-coated dishes (BD Biosciences) in CFU-Hill medium (Stem Cell Technologies, cat#05900). Non-adherent cells were collected 48 hours later, and 1 million cells seeded on 24 well Fibronectin-coated dishes. On day 5, EPC-CFUs were counted in 5 wells. The mean EPC-CFU was used in analyses as previously described [14 (link), 39 (link)].

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Martinez C.A., Rikhi R., Pester M.S., Parker M., Gonzalez A., Larson M., Chavez J., Mendez A., Raines J.K., Kolber M.A., Schulman I.H., Alcaide M.L, & Hurwitz B.E. (2022). Abacavir antiretroviral therapy and indices of subclinical vascular disease in persons with HIV. PLoS ONE, 17(3), e0264445.

Publication 2022

Ficoll Paque Fibronectin-coated dishes CFU-Hill medium

Blood Cells Dishes Fibronectin Ficoll Stem cell

Corresponding Organization : University of Miami

Other organizations : Wake Forest University

Top 5 similar protocols

Variable analysis

independent variables

Isolation of early EPCs using Ficoll Paque
Seeding 5 million cells on 6-well Fibronectin-coated dishes in CFU-Hill medium
Collecting non-adherent cells 48 hours later
Seeding 1 million non-adherent cells on 24 well Fibronectin-coated dishes

dependent variables

EPC-CFU count on day 5

control variables

Volume of intravenous blood (45 mL)
Fibronectin-coated dishes (BD Biosciences)
CFU-Hill medium (Stem Cell Technologies, cat#05900)

positive controls

Not specified

negative controls

Not specified

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