Characterization of Cancer Cell Lines

The ovarian cancer cell lines SKOV3 (p53^Null/KRAS^Wt), A2780 (p53^Wt/KRAS^Wt), and HEY-A8 (p53^Wt/KRAS^G12D) 16 were either purchased from the American Type Culture Collection (ATCC) or maintained in our laboratory with Roswell Park Memorial Institute (RPMI) 1640 medium (Solarbio Life Sciences, Shanghai). The lung cancer cell lines A549 (p53^Wt/KRAS^Mt), H358(p53^Null/KRAS^Mt), H23 (p53^Mt/KRAS^Mt) and H1299 (p53^Null/KRAS^Wt) 17 (link), 18 (link); the retroviral packaging cells (Phoenix amphotropic cells); and the lentiviral packaging cells (293 T cells) were also purchased from ATCC and were maintained in Dulbecco's modified Eagle's medium (DMEM; HyClone). All cell media were supplemented with 10% fetal bovine serum, 2 mM L-glutamine, penicillin (100 units/ml) and streptomycin (100 μg/ml), and cells were cultured in a humidified incubator at 37°C with 5% CO₂.