Gamma scintigraphy was carried out to confirm the development of sustained release depot at the site of injection following the administration of liquid ZP-LPS formulation. Briefly, ZP was radiolabeled with 99mTechnetium-pertechnetate (Tc-99M) by an in-house developed standard procedure using stannous chloride as reducing agent. Labeling efficiency and stability was confirmed by instant thin layer chromatography.16 (link) ZP-LPS Formulation was then developed using Tc-99M labeled ZP. ZP-LPS formulation (0.3 mL) containing 60 μCi ofTc-99Mwas then injected subcutaneously at the back of the three Sprague-Dawley rats. At pre-designated time points, 15 min, 6 h and 12 h, gamma images were captured by gamma camera (Siemens T2 SPECT-CT) to understand drug release and depot formation.17 (link)
Protocol detail
Gamma Scintigraphy for Sustained Release Depot Evaluation
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Drug release
Gamma
Gamma camera
Pertechnetate
Reducing agent
Scintigraphy
Spect ct
Sprague dawley rats
Stannous chloride
Tc 99m
Thin layer chromatography
Corresponding Organization : Jamia Hamdard
Top 5 similar protocols
Variable analysis
independent variables
- Administration of liquid ZP-LPS formulation
- Development of sustained release depot at the site of injection
- Drug release and depot formation
- Tc-99M labeling of ZP using stannous chloride as reducing agent
- Confirmation of labeling efficiency and stability by instant thin layer chromatography
- Positive control: Tc-99M labeled ZP
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!