Baclight viability dyes (Invitrogen) were used to assess Gc survival intracellularly and extracellularly in the presence of PMNs as described (Criss et al., 2009 (link)). In some experiments, PMNs were treated with 1x Protease Inhibitor Cocktail Set V containing AEBSF, aprotinin, E-64, and leupeptin (Calbiochem) during attachment to coverslips and throughout the infection.
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Assessing Gc Survival with Baclight
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Other organizations : University of Virginia
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Variable analysis
independent variables
- Treatment of PMNs with 1x Protease Inhibitor Cocktail Set V containing AEBSF, aprotinin, E-64, and leupeptin (Calbiochem) during attachment to coverslips and throughout the infection
- Gc survival intracellularly
- Gc survival extracellularly
- Attachment of PMNs to coverslips
- Infection conditions
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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