If not indicated otherwise, C. albicans strain SC5314 was used throughout all experiments. For studies on Tye7, strain SN152 tye7Δ/Δ and the corresponding wild-type strain SN152 were used (Noble and Johnson, 2005 (link); Homann et al., 2009 (link)). Pre-cultures were grown over night at 30°C in 20 ml YPD medium (per liter: 10 g yeast extract, 20 g peptone, and 20 g glucose). Cells were harvested by centrifugation at 4000 ×g, washed twice in phosphate-buffered saline (PBS) and suspended in either YPD or MOPS-buffered RPMI 1640 (Sigma) with 2% glucose (per liter: 10.4 g RPMI 1640, 34.53 g MOPS, 20 g glucose; pH 6.8; subsequently defined as RPMI medium). Stock solution of caspofungin (5 mg/ml; Cancidas®, Merck, Germany), flucytosine (10 mg/ml), cycloheximide (20 mg/ml; Sigma C7698) or sodium azide (20 mg/ml) were prepared in PBS and filter sterilized. Amphotericin B stock solution was prepared at 4 mg/ml in DMSO. Fluconazole solution at 2 mg/ml was purchased from B. Braun, Germany. All drugs were diluted in the respective media that were used for sensitivity analyses. Controls were prepared according to the solvents used for stock solutions of the respective antibiotics.
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