Culturing Toxoplasma gondii and Cell Lines

All T. gondii strains were maintained in Vero cells in RPMI (Nacalai Tesque) supplemented with 2% heat-inactivated FBS (JRH Bioscience), 100 U/ml penicillin, and 0.1 mg/ml streptomycin (Nacalai Tesque), as previously described (55 (link)). HAP1 cells were maintained in IMDM (Nacalai Tesque) containing 10% heat-inactivated FBS, and 100 U/ml penicillin, and 0.1 mg/ml streptomycin. HFFs, Huh7 cells were maintained in RPMI (Nacalai Tesque) containing 10% heat-inactivated FBS, and 100 U/ml penicillin, and 0.1 mg/ml streptomycin. HeLa cells and MEF cells were maintained in DMEM (Nacalai Tesque) containing 10% heat-inactivated FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin.