Bak Oligomerization Assay for Mitochondrial Analysis

The Bak oligomerization assay was performed as previously reported47 (link). Briefly, mitochondria were isolated and incubated with 4 mM disuccinimidyl suberate (Sigma, St. Louis, Missouri, USA) for 30 min at room temperature. Cross-linked samples were analyzed by western blotting using an anti-Bak antibody (Epitomics, Burlingame, California, USA). Rabbit anti-VDAC1 monoclonal antibodies (Abcam, Cambridge, Massachusetts, USA) were used as a mitochondrial loading control.

Free full text: Click here

Chen C., Zhao D., Fang S., Chen Q., Cheng B., Fang X, & Shu Q. (2017). TRIM22-Mediated Apoptosis is Associated with Bak Oligomerization in Monocytes. Scientific Reports, 7, 39961.

Publication 2017

disuccinimidyl suberate anti-Bak antibody

Anti antibodies Anti antibody Assay Disuccinimidyl suberate Mitochondria Mitochondrial Monoclonal antibodies Rabbit Vdac1

Corresponding Organization : Children's Hospital of Zhejiang University

Other organizations : Zhejiang University

Top 5 similar protocols

Variable analysis

independent variables

Mitochondria were incubated with 4 mM disuccinimidyl suberate (Sigma, St. Louis, Missouri, USA) for 30 min at room temperature

dependent variables

Cross-linked samples were analyzed by western blotting using an anti-Bak antibody (Epitomics, Burlingame, California, USA)

control variables

Rabbit anti-VDAC1 monoclonal antibodies (Abcam, Cambridge, Massachusetts, USA) were used as a mitochondrial loading control

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!