Bone marrow-derived DCs (2x105) were transfected with pMAC/PS-scFv (20 μg/mL) using JetPEI Macrophage (Polyplus Transfection). DCs transfected with an empty vector were used as a control (pMAC/PS). The empty pMAC/PS vector and the vector encoding the scFv were obtained as previously described [12 (link)].
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