Vero Cell Cytotoxicity Assay for TB Compounds

Vero cells (ATCC CCL-81) were used to test cytotoxicity as described previously (18 (link)). Briefly, Vero cells were plated at a concentration of 10⁵ cells/well in 96-well plates and incubated for 2 to 3 h to allow the cells to settle. Test compounds were serially diluted separately in Eagle’s minimal essential medium to generate test concentrations ranging from 200 to 0.1× M. tuberculosis MIC. The serial dilutions were then added to the plated cells and incubated for 48 h at 37°C. The viability of Vero cells exposed to each compound was determined using an MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] cell viability kit (Promega).