Tyramide Signal Amplification Immunostaining

Single and dual antibody staining using tyramide signal amplification (ThermoFisher) were performed on formalin-fixed, paraffin-embedded skin samples as previously described (18 (link)). Antibodies were all purchased from Sigma. Immunohistochemistry images were obtained with an Evos FL Auto microscope (Life Technologies). Confocal images were captured on an Olympus Fluoview 1000 confocal microscope using an oil immersion 100X objective.

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Gaydosik A.M., Tabib T., Geskin L.J., Bayan C.A., Conway J.F., Lafyatis R, & Fuschiotti P. (2019). Single-cell lymphocyte heterogeneity in advanced Cutaneous T-Cell Lymphoma skin tumors. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(14), 4443-4454.

Publication 2019

tyramide signal amplification Evos FL Auto microscope Fluoview 1000 confocal microscope

Antibodies Antibody Confocal microscope Embedded paraffin Formalin Immersion Immunohistochemistry Microscope Skin

Corresponding Organization : University of Pittsburgh

Other organizations : Columbia University Irving Medical Center

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Variable analysis

independent variables

Antibody staining method (single and dual antibody staining)

dependent variables

Immunohistochemistry and confocal microscopy images of skin samples

control variables

Formalin-fixed, paraffin-embedded skin samples
Antibodies purchased from Sigma
Microscopes used (Evos FL Auto and Olympus Fluoview 1000 confocal)

controls

Positive and negative controls not explicitly mentioned

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