Characterization of Canine Cardiac Progenitor Cells

Canine CDCs were characterized by flow cytometry as described 7 (link),10 (link). Flow cytometry was performed on canine CDCs using a FACScalibur and LSR II (BD Biosciences, San Jose, CA, USA) and analysed using FlowJo software (TreeStar, Ashland, OR, USA). Cells were incubated with antibodies against CD105 (ab156756; Abcam, Cambridge, England), CD90 (bd555595; BD Biosciences), CD45 (mca1042a488; AbD Serotec, Kidlington, United Kingdom), CD117 (c-kit; ab5631; Abcam) for 60 min. Isotype-identical antibodies served as negative controls.

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Hensley M.T., de Andrade J., Keene B., Meurs K., Tang J., Wang Z., Caranasos T.G., Piedrahita J., Li T.S, & Cheng K. (2015). Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts. Journal of Cellular and Molecular Medicine, 19(8), 1805-1813.

Publication 2015

FACScalibur ab156756 bd555595 ab5631

Antibodies C kit Canine Cd90 Cdcs Cells Flow cytometry Isotype

Corresponding Organization : University of North Carolina at Chapel Hill

Other organizations : First Affiliated Hospital of Zhengzhou University, Nagasaki University

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Variable analysis

independent variables

Antibodies used for flow cytometry: CD105, CD90, CD45, CD117 (c-kit)

dependent variables

Expression of cell surface markers CD105, CD90, CD45, CD117 (c-kit) on canine CDCs

control variables

Incubation time for antibody staining: 60 min
Flow cytometry instruments used: FACScalibur and LSR II (BD Biosciences)
Software used for data analysis: FlowJo (TreeStar)

controls

Isotype-identical antibodies served as negative controls

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