Pharmacokinetic Study of Drugs Using Rat Liver S9 Fraction

ACS/HPLC reagent-grade acetonitrile, methanol, and water were procured from Honeywell (Morristown, NJ, USA). HPLC-grade dimethyl sulfoxide (DMSO) and formic acid (FA) were procured from Junsei Chemical Co., Ltd. (Tokyo, Japan) and Dae-Jung Chemicals and Metals Co., Ltd. (Siheung, Republic of Korea). Buspirone (BUS), dextromethorphan (DEX), diclofenac (DIC), and alpelisib (IS) were procured from MedKoo Bioscience, Inc. (Morrisville, NC, USA). Magnesium Chloride (MgCl₂), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), polyethylene glycol 400 (PEG400), and potassium phosphate buffer were procured from Sigma-Aldrich Co. (St. Louis, MO, USA). Sprague Dawley (SD) rat liver S9 fraction was procured from Sekisui Xenotech (Kansas City, KS, USA). SD rats (Male; nine-week-old; weighing range: 250–270 g) were supplied by Koatech Co. (Pyeongtaek, Republic of Korea). The SD rats were used for pharmacokinetic studies after an acclimation period of 7 days. Feeding, excluding water, was restricted for 12 h before starting the pharmacokinetics study. All animal studies were conducted with approval from the Institutional Animal Care and Use Committee of Pusan National University (approval number: PNU-2023-3245).

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Keem M.J., Seo S.W., Kim T., Jo B.G., Kim S.N., Yoon I.S, & Yang M.H. (2023). A High-Performance Liquid Chromatography with Photodiode Array Detection Method for Simultaneous Determination of Three Compounds Isolated from Wikstroemia ganpi: Assessment of the Effects on Cytochrome P450-Mediated Metabolism In Vitro and In Vivo. Nutrients, 15(18), 4061.

Publication 2023

methanol Magnesium Chloride (MgCl2), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), polyethylene glycol 400 (PEG400), potassium phosphate buffer SD rats

Acclimation Acetonitrile Alpelisib Animal Buffer Buspirone Dextromethorphan Diclofenac Dimethyl sulfoxide Formic acid Hplc Institutional animal care and use committee Liver Magnesium chloride Male Metals Methanol Nadph Pharmacokinetics Polyethylene glycol 400 Potassium phosphate Salt Sprague dawley rats

Corresponding Organization :

Other organizations : Pusan National University, Korea Institute of Science and Technology

Top 5 similar protocols

Variable analysis

independent variables

Buspirone (BUS)
Dextromethorphan (DEX)
Diclofenac (DIC)
Alpelisib (IS)

dependent variables

Pharmacokinetic parameters

control variables

ACS/HPLC reagent-grade acetonitrile, methanol, and water
HPLC-grade dimethyl sulfoxide (DMSO) and formic acid (FA)
Magnesium Chloride (MgCl2)
β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH)
Polyethylene glycol 400 (PEG400)
Potassium phosphate buffer
Sprague Dawley (SD) rat liver S9 fraction
SD rats (Male; nine-week-old; weighing range: 250–270 g)

positive controls

Alpelisib (IS)

negative controls

Not explicitly mentioned

Annotations

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