Bacterial Culture Conditions for Diverse Microbes

H. pylori strains 26695, G27, 98-10, and isogenic 26695 mutants (19 (link), 20 (link)) were grown on Trypticase soy agar supplemented with 5% sheep blood (BD Biosciences) or brucella broth supplemented with 5% fetal bovine serum (FBS) at 37°C in 5% atmosphere CO₂. E. coli MG1655 harboring the conjugative plasmid pKM101, E. coli MS411 harboring the R1-16 conjugative plasmid, and E. coli WM1652 recipient cells were grown on lysogeny broth (LB) agar or broth supplemented with ampicillin (50 µg/ml), kanamycin (50 µg/ml), or tetracycline (20 µg/ml), respectively. Agrobacterium tumefaciens C58 and its derivative GV3101 were maintained on LB plates supplemented with rifampin (25 µg/ml) at 28°C. A. tumefaciens GV3101 harboring pCAMBIA vectors were maintained on LB containing rifampin (25 µg/ml) and kanamycin (100 µg/ml).